Exchange Program Brazil-US – PVE
December, 07-20, 2023.
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Monday |
Tuesday |
Wednesday |
Thursday |
Friday |
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Dec 07, 14-17 hs |
Dec 8, 10-12 hs 14-16 hs |
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Dec 11, 14-17 hs |
Dec 12, 10-12 hs 14-17 hs |
Dec 13, 10-12 hs 14-17 hs |
Dec 14, 10-12 hs 14-17 hs |
Dec 15, 10-12 hs 14-16 hs |
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Dec 18, 14-17 hs |
Dec 19, 10-12 hs 14-17 hs |
Dec 20, 10-12 hs |
Dr. Vitor Serrão
University of California Santa Cruz
Establishment of a high-throughput single-particle cryoEM analysis workflow
Day 1 (Dec 07, 2023): Introduction: Structural biology, an overview and New advances in structural biology, mainly cryogenic transmission electron microscopy. Day 2 (Dec 08, 2023): Introduction to electron microscopy. Applications to Life and Materials Sciences: Advances in the transmission electron microscopy field, including cryo-electron tomography and micro-electron diffraction
Hands-on 1: Preparing samples at room-temperature (negatively stained) and cryogenic conditions (vitrification) at the Crystallography Group – IFSC. Day 3 (Dec 11, 2023): Data collection: Theory and methods description.
Hands-on 2: Hands-on in remote data collection
Day 4 (Dec 12, 2023): Images and Transforms, filtering, CTF, and initial steps in processing
Day 5 (Dec 13, 2023): Principles of single particle analysis
Hands-on 4: Hands-on in remote data analysis
Day 6 (Dec 14, 2023): Bayesian methods, particle classification
Hands-on 5: Hands-on in remote data analysis
Day 7 (Dec 15, 2023): Fourier shell correlation and map validation
Hands-on 6: Hands-on in remote data analysis
Day 8 (Dec 18, 2023): Cryo-Electron tomography and subtomogram averaging applied in life sciences
Hands-on 8: Hands-on in a real dataset
Day 9 (Dec 19, 2023): New advances in cryo-EM
Roundtable discussion: Integrated, open software for science: advantages and challenges
Day 10 (Dec 20, 2023): Students’ seminars and presentations
INSCRIÇÕES:
Os estudantes interessados deverão se inscrever na disciplina SFI5898 - Seminário de Pós-Graduação II, no período de 13 a 24/11/2023, através dos formulários online nos seguintes links:
- Alunos regulares de pós-graduação da USP, devem usar esse formulário: https://forms.gle/8K3f1RLnvjB4mjFa6
- Alunos ativos de graduação da USP, alunos de pós-graduação externos à USP ou graduados de qualquer IES, devem usar esse formulário: https://forms.gle/QD2xTB6NmuRDJL2F6
Realização Apoio
http://www.ifsc.usp.br/comissaopq/minicurso
Contact: Prof. Otavio Henrique Thiemann (
CEP: 09913-030 Diadema - São Paulo / SP